Quantifying Cell Death Induced by the NLRC4 Inflammasome

The Nod-like Receptor (NLR) apoptosis inhibitory proteins (NAIPs) are cytosolic receptors that sense cytosolic bacterial proteins. NAIP ligation induces its association with NLRC4, leading to the assembly of the NAIP/NLRC4 inflammasome, which induces the activation of the caspase-1 protease. Caspase...

Full description

Saved in:
Bibliographic Details
Published inMethods in molecular biology (Clifton, N.J.) Vol. 2696; p. 199
Main Authors Emming, Stefan, Monteleone, Mercedes M, Kambara, Hiroto, Starchenko, Alina, Alley, Jennifer, Nolan, Michael A, Li, Wei, Kilty, Iain, Schroder, Kate
Format Journal Article
LanguageEnglish
Published United States 2023
Subjects
Animals
Apoptosis Regulatory Proteins - metabolism
Calcium-Binding Proteins - metabolism
CARD Signaling Adaptor Proteins - metabolism
Caspase 1 - metabolism
Caspases - metabolism
Cell Death
Humans
Inflammasomes - metabolism
Mice
Flatox
Needletox
Pyroptosis
Cell death
Inflammasome
NLRC4
Macrophages
NAIP
Online AccessGet more information

Cover

More Information
Summary:The Nod-like Receptor (NLR) apoptosis inhibitory proteins (NAIPs) are cytosolic receptors that sense cytosolic bacterial proteins. NAIP ligation induces its association with NLRC4, leading to the assembly of the NAIP/NLRC4 inflammasome, which induces the activation of the caspase-1 protease. Caspase-1 then cleaves pro-interleukin (IL)-1β, pro-IL-18, and gasdermin D and induces a form of pro-inflammatory cell death, pyroptosis. These processes culminate in host defense against bacterial infection. Here we describe methods for activating NAIP/NLRC4 inflammasome signalling in human and murine macrophages and quantifying inflammasome-induced cell death.
ISSN:1940-6029
DOI:10.1007/978-1-0716-3350-2_13