Expression and Functional Analysis of Ctenophore Glutamate Receptor Genes

• Published in: Methods in molecular biology (Clifton, N.J.) Vol. 2757; p. 259
• Main Author: Mayer, Mark L
• Format: Journal Article
• Language: English
• Published: United States 2024
• Subjects: Animals; Ctenophora - genetics; Ctenophora - metabolism; Gene Expression Profiling - methods; Immunohistochemistry; Receptors, Glutamate - genetics; Receptors, Glutamate - metabolism; Transcriptome - genetics; Glutamate receptors; Xenopus oocytes; Ion channels; Voltage clamp
• ISSN: 1940-6029
• DOI: 10.1007/978-1-0716-3642-8_11

The functional analysis of ctenophore neurotransmitter receptors, transporters, and ion channels can be greatly simplified by use of heterologous expression systems. Heterologous expression allows the characterization of individual membrane proteins, expressed at high levels in cells, where background activity by endogenous ion channels and transporters is with few exceptions minimal. The goal of such experiments is to gain an in-depth understanding of the behavior and regulation of individual molecular species, which is challenging in native tissue, but especially so in the case of ctenophores and other marine organisms. Coupled with transcriptome analysis, and immunohistochemical studies of receptor expression in vivo, experiments with heterologous expression systems can provide valuable insight into cellular activity, prior to more challenging functional studies on native tissues.