Cytometry in High-Containment Laboratories

• Published in: Methods in molecular biology (Clifton, N.J.) Vol. 2779; p. 425
• Main Authors: Cohen, Melanie, Laux, Julie, Douagi, Iyadh
• Format: Journal Article
• Language: English
• Published: United States 2024
• Subjects: Artificial Intelligence; Biomedical Research; Containment of Biohazards; Laboratories; Technology; High containment; Flow cytometry; Infectious sample; Multiomics; Cell sorting; CYTOF; Cell analysis; Biosafety; Cytometry; Infectious disease; Aerosols; Public health; Emerging pathogens
• DOI: 10.1007/978-1-0716-3738-8_20

The emergence of new pathogens continues to fuel the need for advanced high-containment laboratories across the globe. Here we explore challenges and opportunities for integration of cytometry, a central technology for cell analysis, within high-containment laboratories. We review current applications in infectious disease, vaccine research, and biosafety. Considerations specific to cytometry within high-containment laboratories, such as biosafety requirements, and sample containment strategies are also addressed. We further tour the landscape of emerging technologies, including combination of cytometry with other omics, the application of automation, and artificial intelligence. Finally, we propose a framework to fast track the immersion of advanced technologies into the high-containment research setting to improve global preparedness for new emerging diseases.