Drug resistance mutation of HIV-1 in HIV/AIDS patients infected by blood transfusion

• Published in: Jie fang jun yi xue za zhi Vol. 38; no. 3; p. 230
• Main Authors: Xin-li, LU, Hong-ru, ZHAO, Yu-qi, ZHANG, Cui-ying, ZHAO, Han-ping, LI, Guang-yi BAI, Qiao-min, LI, CHEN, Zhi-qiang, Bao-jun, LI, Li-hua, LIU
• Format: Journal Article
• Language: Chinese; English
• Published: Beijing People's Military Medical Press 01.01.2013
• Subjects: Acquired immune deficiency syndrome; AIDS; Antiretroviral drugs; Mutation; Protease inhibitors
• ISSN: 0577-7402

Objective 　To study the characteristic of HIV-1 gene mutation in HIV/AIDS patients infected by blood transfusion, and analyze the resistance to anti-HIV drugs. Methods 　Plasma samples were collected from 37 HIV/AIDS patients infected by blood transfusion for extraction of HIV-1 RNA. The gene fragments of HIV pol domain were amplified by RT-PCR and nested-PCR , and the electrophoresis positive products were sequenced. The sequencing result was landed to the website http:// HIV-1db.stanford.edu to analyze the drug resistance mutations. Results 　Drug resistance mutations were found in 20 patients, including 19 cases of virological or immunological failure. Mutation of gene locus V32AV of protease inhibitors (PIs) occurred in 3 patients during the treatment, but it did not cause the drug resistance of PIs. Mutation of the coding regions of reverse transcriptase was found in 23 patients, including M184V, TAMs, Q151M complexus, K103N, Y181C and so on. Of the 23 patients mentioned above, the HIV-1 gene mutation induced the resistance to reverse transcriptase inhibitors (RTIs) in 20 patients, and the mutation rate of RTIs was 54.05% (20/37). Conclusion 　The drug resistance rate of HIV-1 in patients infected by blood transfusion may be high for antiviral therapy, so the drug resistance of HIV-1 should be monitored and treatment plan should be adjusted timely.