Isolation of Single Circulating Tumor Cells Using VyCAP Puncher System

Tumor heterogeneity has a major role in the development of tumor evasion and resistance to treatments. To study and understand the intrinsic heterogeneity of cancer cells, the use of single-cell isolation technology has had a major boost in recent years, gaining ground to bulk analysis in the study...

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Published inMethods in molecular biology (Clifton, N.J.) Vol. 2752; p. 65
Main Authors Pereira-Veiga, Thais, Behrens, Bianca, Broekmaat, Joska J, Oomens, Lisa, Stevens, Michiel, Tibbe, Arjan G J, Stoecklein, Nikolas, Muinelo-Romay, Laura, Piñeiro, Roberto, Costa, Clotilde
Format Journal Article
LanguageEnglish
Published United States 2024
Subjects
Cell Separation
Humans
Liquid Biopsy
Neoplastic Cells, Circulating
Single-Cell Analysis
Technology
CTC
Single cell
Tumor heterogeneity
Liquid biopsy
VyCAP
CellSearch
Puncher
Sequencing
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Summary:Tumor heterogeneity has a major role in the development of tumor evasion and resistance to treatments. To study and understand the intrinsic heterogeneity of cancer cells, the use of single-cell isolation technology has had a major boost in recent years, gaining ground to bulk analysis in the study of solid tumors. In the liquid biopsy field, the use of technologies for single-cell analysis has represented a major advance in the study of the heterogeneity of circulating tumor cells (CTCs), providing relevant information about therapy-resistant CTCs. However, single-cell analysis of CTCs is still challenging due to the weakness and scarcity of these cells. In this chapter, we describe a protocol for CTCs isolation at a single-cell level using the VyCAP Puncher system.
ISSN:1940-6029
DOI:10.1007/978-1-0716-3621-3_5