Isolation of Extracellular Vesicles Using Formulas to Adapt Centrifugation to Different Centrifuges

Extracellular vesicles (EVs) are small lipid bilayer vesicles released by cells to facilitate cell-to-cell communication. To study their biological roles and functions, they need to be isolated and purified, which can be achieved through a variety of methods. Here, we describe different methods for...

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Bibliographic Details
Published inMethods in molecular biology (Clifton, N.J.) Vol. 2761; p. 39
Main Authors Teles, Ramon Handerson Gomes, Engelmayr, Daniela, Meybohm, Patrick, Burek, Malgorzata
Format Journal Article
LanguageEnglish
Published United States 2024
Subjects
Centrifugation
Centrifugation, Density Gradient - methods
Extracellular Vesicles
Biomarker
Breast cancer
Exosomes
Centrifugation
Extracellular vesicles isolation
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Summary:Extracellular vesicles (EVs) are small lipid bilayer vesicles released by cells to facilitate cell-to-cell communication. To study their biological roles and functions, they need to be isolated and purified, which can be achieved through a variety of methods. Here, we describe different methods for isolating and purifying EVs, with a focus on calculating the required g-force and centrifugation time with different centrifuges and rotors. We have compiled key formulas and tested predicted parameters for EV acquisitions to provide a comprehensive guide for EV isolation.
ISSN:1940-6029
DOI:10.1007/978-1-0716-3662-6_3