Detection and Discovery of Coronaviruses in Wild Bird Populations

Wild birds are natural hosts of multiple microbial agents, including a wide diversity of coronaviruses. Here we describe a pan-Coronavirus detection RT-PCR method to identify those viruses regardless of the coronavirus genus or nature of the specimen. We also describe a protocol using high-throughpu...

Full description

Saved in:
Bibliographic Details
Published inMethods in molecular biology (Clifton, N.J.) Vol. 2203; p. 41
Main Authors Snoeck, Chantal J, Zohari, Siamak
Format Journal Article
LanguageEnglish
Published United States 2020
Subjects
Animals
Animals, Wild
Bird Diseases - virology
Coronavirus - genetics
Coronavirus - isolation & purification
Coronavirus Infections - genetics
Coronavirus Infections - veterinary
Real-Time Polymerase Chain Reaction - methods
RNA-Dependent RNA Polymerase - genetics
Specimen Handling - methods
Deltacoronavirus
Sample collection
Gammacoronavirus
Wild birds
Next-generation sequencing
Illumina MiSeq
Pan-Coronavirus
RT-PCR
Online AccessGet more information

Cover

More Information
Summary:Wild birds are natural hosts of multiple microbial agents, including a wide diversity of coronaviruses. Here we describe a pan-Coronavirus detection RT-PCR method to identify those viruses regardless of the coronavirus genus or nature of the specimen. We also describe a protocol using high-throughput sequencing technologies to obtain their entire genome, which overcomes the inherent difficulties of wild bird coronavirus sequencing, that is, their genetic diversity and the lack of virus isolation methods.
ISSN:1940-6029
DOI:10.1007/978-1-0716-0900-2_3