Immunostaining for Epigenetic Modifications in Fagopyrum Calli

Immunostaining is a well-established technique for identifying specific proteins in tissue samples with specific antibodies to identify a single target protein. It is commonly used in research and provides information about cellular localization and protein expression levels. This chapter describes...

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Bibliographic Details
Published inMethods in molecular biology (Clifton, N.J.) Vol. 2791; p. 15
Main Authors Tomasiak, Alicja, Sala, Katarzyna, Braszewska, Agnieszka
Format Journal Article
LanguageEnglish
Published United States 2024
Subjects
Callus
Antibody
Histone methylation
DNA methylation
Fagopyrum
Histone acetylation
Immunostaining
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Summary:Immunostaining is a well-established technique for identifying specific proteins in tissue samples with specific antibodies to identify a single target protein. It is commonly used in research and provides information about cellular localization and protein expression levels. This chapter describes a detailed protocol for immunostaining fixed Fagopyrum calli embedded in Steedman's wax using nine antibodies raised against histone H3 and H4 methylation and acetylation on several lysines and DNA methylation.
ISSN:1940-6029
DOI:10.1007/978-1-0716-3794-4_2