An enzyme-linked immunoassay for circulating immune complexes using solid phased goat Clq

An ELISA procedure was developed for measuring circulating immune complexes (IC), using solid phased goat Clq. The use of purified Clq from this species significantly diminished the background uptake of the enzyme-labeled goat antibodies used in the assay, in comparison with Clq isolated from human,...

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Bibliographic Details
Published inJournal of immunological methods Vol. 63; no. 2; p. 187
Main Authors Lin, T M, Halbert, S P, Cort, R, Blaschke, M J
Format Journal Article
LanguageEnglish
Published Netherlands 14.10.1983
Subjects
Adult
Animals
Antigen-Antibody Complex - analysis
Arthritis, Rheumatoid - immunology
Binding, Competitive
Complement Activating Enzymes
Complement C1q
DNA - pharmacology
Edetic Acid - pharmacology
Enzyme-Linked Immunosorbent Assay
Epitopes
Goats
Guinea Pigs
Humans
Immunoenzyme Techniques
Immunoglobulin G - analysis
Lupus Erythematosus, Systemic - immunology
Rabbits
Reference Standards
Toxoplasmosis - immunology
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Summary:An ELISA procedure was developed for measuring circulating immune complexes (IC), using solid phased goat Clq. The use of purified Clq from this species significantly diminished the background uptake of the enzyme-labeled goat antibodies used in the assay, in comparison with Clq isolated from human, guinea pig or rabbit serum. The test specimen results are reported as micrograms equivalent (microgram eq)/ml of heat aggregated human immunoglobulin G (HAIgG), and are based on standard curves developed with this latter reagent for each assay. The 2 World Health Organization (WHO) reference preparations for immune complex determinations (HAIgG and a human tetanus antitoxin-toxoid immune complex) were assayed by this ELISA procedure, and the results obtained were in very good agreement with the WHO established values. All the reagents in the ELISA, including the lyophilized HAIgG standard and the solid phased Clq are stable for more than one year at 4 degrees C. The range of accurate quantitation in test serum is 2-500 micrograms/ml, when using a 1:100 specimen dilution. The total incubation is less than 2 h, with no preliminary preparation of test specimens. The average concentration of IC reactivity in 126 healthy adults was 6 micrograms eq/ml, and the normal upper limit was determined to be 12 micrograms eq/ml.
ISSN:0022-1759