Hexaammineruthenium as an electron donor to mitochondrial cytochrome oxidase: membrane potential generation in the absence of cytochrome c

• Published in: Biochemistry international Vol. 12; no. 1; p. 103
• Main Authors: Tsofina, L M, Liberman, E A, Vygodina, T V, Konstantinov, A A
• Format: Journal Article
• Language: English
• Published: Australia 01.01.1986
• Subjects: Animals; Cytochrome c Group - metabolism; Electron Transport Complex IV - metabolism; Kinetics; Membrane Potentials - drug effects; Mitochondria, Liver - enzymology; Rats; Ruthenium - metabolism; Ruthenium - pharmacology; Ruthenium Compounds

Cytochrome c oxidase can generate membrane potential in the absence of cytochrome c (e.g., in cytochrome c-deficient mitochondria or in proteoliposomes) with hexaammineruthenium as an artificial electron donor. Of several other redox mediators tested, phenazine methosulfate was found to be an effici...