Hexaammineruthenium as an electron donor to mitochondrial cytochrome oxidase: membrane potential generation in the absence of cytochrome c

Cytochrome c oxidase can generate membrane potential in the absence of cytochrome c (e.g., in cytochrome c-deficient mitochondria or in proteoliposomes) with hexaammineruthenium as an artificial electron donor. Of several other redox mediators tested, phenazine methosulfate was found to be an effici...

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Bibliographic Details
Published inBiochemistry international Vol. 12; no. 1; p. 103
Main Authors Tsofina, L M, Liberman, E A, Vygodina, T V, Konstantinov, A A
Format Journal Article
LanguageEnglish
Published Australia 01.01.1986
Subjects
Animals
Cytochrome c Group - metabolism
Electron Transport Complex IV - metabolism
Kinetics
Membrane Potentials - drug effects
Mitochondria, Liver - enzymology
Rats
Ruthenium - metabolism
Ruthenium - pharmacology
Ruthenium Compounds
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Summary:Cytochrome c oxidase can generate membrane potential in the absence of cytochrome c (e.g., in cytochrome c-deficient mitochondria or in proteoliposomes) with hexaammineruthenium as an artificial electron donor. Of several other redox mediators tested, phenazine methosulfate was found to be an efficient artificial substrate for membrane energization by cytochrome oxidase, whereas TMPD, DAD, DCPIP or ferrocyanide are virtually ineffective. The ability of Ru(NH3)6(2+) and phenazine methosulfate to support the generation of delta psi by cytochrome c-oxidase correlates with their effectiveness as electron donors to cytochrome a in the cyanide-inhibited membrane-bound enzyme.
ISSN:0158-5231