Detection of reverse transcriptase activity in association with the non-A, non-B hepatitis agent(s)

Particle-associated reverse transcriptase activity was detected in four human serum specimens and in two plasma-derived products, all of which had been shown to transmit non-A, non-B hepatitis (NANBH) to other human beings and/or chimpanzees. Reverse transcriptase activity was also detected in all t...

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Bibliographic Details
Published inThe Lancet (British edition) Vol. 2; no. 8409; p. 941
Main Authors Seto, B, Coleman, Jr, W G, Iwarson, S, Gerety, R J
Format Journal Article
LanguageEnglish
Published England 27.10.1984
Subjects
Animals
Centrifugation, Density Gradient
Hepatitis C - enzymology
Hepatitis C - microbiology
Hepatitis, Viral, Human - enzymology
Humans
Pan troglodytes
RNA-Directed DNA Polymerase - blood
Virion
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Summary:Particle-associated reverse transcriptase activity was detected in four human serum specimens and in two plasma-derived products, all of which had been shown to transmit non-A, non-B hepatitis (NANBH) to other human beings and/or chimpanzees. Reverse transcriptase activity was also detected in all twelve sera from patients with acute or chronic NANBH. In contrast, reverse transcriptase activity was found in only 2 of 49 serum specimens from healthy plasma donors and laboratory workers. Sucrose density gradient fractions of two of the infectious human sera (peak reverse transcriptase activity at 1.14 g/ml) transmitted NANBH to chimpanzees. Biochemical and enzymatic data indicate that the NANBH agent(s) is a retrovirus or is retrovirus-like.
ISSN:0140-6736