Detection of double-stranded RNA elements in the plant pathogenic fungus Rhizoctonia solani

Many species of fungi have been shown to harbor double-stranded RNA (dsRNA) elements. A single fungal isolate of Rhizoctonia solani may have as many as five different dsRNA elements within them. The presence of specific dsRNA elements influence pathogenicity in host plants.

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Bibliographic Details
Published inMethods in molecular biology (Clifton, N.J.) Vol. 508; p. 171
Main Authors Charlton, Nikki D, Tavantzis, Stellos M, Cubeta, Marc A
Format Journal Article
LanguageEnglish
Published United States 2009
Subjects
Clinical Laboratory Techniques
Fungi - genetics
Plant Diseases - genetics
Plant Diseases - microbiology
Reverse Transcriptase Polymerase Chain Reaction - methods
Rhizoctonia - genetics
RNA, Double-Stranded - isolation & purification
RNA, Double-Stranded - physiology
Sensitivity and Specificity
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Summary:Many species of fungi have been shown to harbor double-stranded RNA (dsRNA) elements. A single fungal isolate of Rhizoctonia solani may have as many as five different dsRNA elements within them. The presence of specific dsRNA elements influence pathogenicity in host plants.
ISSN:1064-3745
DOI:10.1007/978-1-59745-062-1_14