The complete enzymic hydrolysis of crosslinked proteins

Procedures used for the complete enzymic hydrolysis of proteins are reviewed. The successful application of complete enzymic hydrolysis in the detection of naturally occurring isopeptide crosslinks and various other types of chemically introduced crosslinks is described. The method may fail if the l...

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Bibliographic Details
Published inAdvances in experimental medicine and biology Vol. 86B; p. 277
Main Authors Milligan, B, Holt, L A
Format Journal Article
LanguageEnglish
Published United States 1977
Subjects
Animals
Aspartic Acid - analysis
Chemical Phenomena
Chemistry
Cystine
Dipeptides - analysis
Disulfides
Glutamates - analysis
Hot Temperature
Hydrolysis
Lysine - analysis
Peptide Hydrolases
Proteins
Wool
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Summary:Procedures used for the complete enzymic hydrolysis of proteins are reviewed. The successful application of complete enzymic hydrolysis in the detection of naturally occurring isopeptide crosslinks and various other types of chemically introduced crosslinks is described. The method may fail if the level of crosslinking is too high, or if crosslinking is accompanied by racemization. Although it is usual to cleave disulphide crosslinks prior to enzymic digestion, the necessity for this in all cases is questioned.
ISSN:0065-2598
DOI:10.1007/978-1-4757-9113-6_19