Nonradioactive northern and Southern analyses from plant samples
Several specific problems are encountered when nonradioactive detection methods are used in conjunction with plant nucleic acids. In this chapter, we describe protocols for the isolation of DNA and RNA from plant leaves and the preparation of probe molecules by either PCR or in vitro transcription w...
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Published in | Methods in molecular biology (Clifton, N.J.) Vol. 353; p. 69 |
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Main Authors | , , |
Format | Journal Article |
Language | English |
Published |
United States
2007
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Subjects | |
Online Access | Get more information |
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Summary: | Several specific problems are encountered when nonradioactive detection methods are used in conjunction with plant nucleic acids. In this chapter, we describe protocols for the isolation of DNA and RNA from plant leaves and the preparation of probe molecules by either PCR or in vitro transcription with different haptens. Furthermore, standard conditions and possible modifications for hybridization and detection of probes are given. |
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ISSN: | 1064-3745 |
DOI: | 10.1385/1-59745-229-7:69 |