Nonradioactive northern and Southern analyses from plant samples

Several specific problems are encountered when nonradioactive detection methods are used in conjunction with plant nucleic acids. In this chapter, we describe protocols for the isolation of DNA and RNA from plant leaves and the preparation of probe molecules by either PCR or in vitro transcription w...

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Bibliographic Details
Published inMethods in molecular biology (Clifton, N.J.) Vol. 353; p. 69
Main Authors Peterhaensel, Christoph, Weier, Dagmar, Lahaye, Thomas
Format Journal Article
LanguageEnglish
Published United States 2007
Subjects
Biotin
Blotting, Northern - methods
Blotting, Southern - methods
Buffers
Cetrimonium Compounds
Digoxigenin
DNA, Plant - genetics
DNA, Plant - isolation & purification
Electrophoresis, Agar Gel
Fluorescein
Guanidines
Molecular Biology - methods
Molecular Probe Techniques
Phenols
Plant Leaves - chemistry
Polymerase Chain Reaction
RNA, Plant - genetics
RNA, Plant - isolation & purification
Transcription, Genetic
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Summary:Several specific problems are encountered when nonradioactive detection methods are used in conjunction with plant nucleic acids. In this chapter, we describe protocols for the isolation of DNA and RNA from plant leaves and the preparation of probe molecules by either PCR or in vitro transcription with different haptens. Furthermore, standard conditions and possible modifications for hybridization and detection of probes are given.
ISSN:1064-3745
DOI:10.1385/1-59745-229-7:69