Isolation and characterization of human embryonic stem cells

The derivation of human embryonic stem (hES) cells is a challenging procedure. The isolation and maintenance of hES is visually and manually complicated, involving mechanical or enzymatic passaging using either collagenase or trypsin. This chapter describes detailed protocols that have been used for...

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Bibliographic Details
Published inMethods in molecular biology (Clifton, N.J.) Vol. 331; p. 55
Main Authors Mitalipova, Maisam, Palmarini, Gail
Format Journal Article
LanguageEnglish
Published United States 2006
Subjects
Biomarkers
Cell Culture Techniques - methods
Cell Culture Techniques - standards
Cell Differentiation
Cell Separation - methods
Cell Separation - standards
Coculture Techniques - methods
Cryopreservation
Fibroblasts - cytology
Humans
Immunohistochemistry
Karyotyping
Laboratories
Pluripotent Stem Cells - cytology
Quality Control
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Summary:The derivation of human embryonic stem (hES) cells is a challenging procedure. The isolation and maintenance of hES is visually and manually complicated, involving mechanical or enzymatic passaging using either collagenase or trypsin. This chapter describes detailed protocols that have been used for the derivation, maintenance, and characterization of hES cells in vitro along with protocols to test their differentiation potential in vivo. When used as a guideline, these protocols will assist researchers in setting up a hES cell laboratory.
ISSN:1064-3745
1940-6029
DOI:10.1385/1-59745-046-4:55