EVIDENCE FOR THE LINKAGE OF THE IGCH LOCUS TO A GENE CONTROLLING THE IDIOTYPIC SPECIFICITY OF ANTI-p-AZOPHENYLARSONATE ANTIBODIES IN STRAIN A MICE

• Published in: The Journal of experimental medicine Vol. 137; no. 1; pp. 22 - 31
• Main Authors: Pawlak, Laura L., Mushinski, Elizabeth B., Nisonoff, Alfred, Potter, Michael
• Format: Journal Article
• Language: English
• Published: The Rockefeller University Press 01.01.1973
• ISSN: 0022-1007; 1540-9538
• DOI: 10.1084/jem.137.1.22

Anti- p -azophenylarsonate (anti-Ar) antibodies elicited in all strain A/J mice tested share one or more idiotypic specificities. These specificities are also found in the anti-Ar antibodies of mice of the closely related strain, AL/N, but not in those of BALB/c mice. Anti-Ar antibodies were elicited in congenic mice in which the IgC H locus of AL/N mice, which controls allotypic markers in the constant regions of heavy chains, had been introgressively backcrossed for nine generations onto a BALB/c background; the mice were then rendered homozygous for the AL/N allotypic determinant. On the average, these antibodies were quantitatively equivalent, with respect to content of the cross-reactive idiotype, to those of AL/N mice. This indicates that the gene controlling the idiotype is closely linked to the IgC H locus. Since idiotype must be a function of V region sequences, the results suggest close linkage of V H and C H genes. The cross-reactive idiotype was found in nearly all F 1 mice (C57/BL x A/J or BALB/c x A/J) tested.