In vitro differentiation of synovial-derived mesenchymal stem cells infected by adenovirus vector mediated by bone morphogenetic protein 2/7 genes into fibrocartilage cells in rabbits

To investigate the feasibility of rabbit synovial-derived mesenchymal stem cells (SMSCs) differentiating into fibrocartilage cells by the recombinant adenovirus vector mediated by bone morphogenetic protein 2/7 (BMP-2/7) genes in vitro. SMSCs were isolated and purified from 3-month-old New Zealand w...

Full description

Saved in:
Bibliographic Details
Published inZhongguo xiu fu chong jian wai ke za zhi Vol. 27; no. 3; p. 345
Main Authors Fu, Peiliang, Zhang, Lei, Wu, Haishan, Cong, Ruijun, Chen, Song, Ding, Zheru, Hu, Kaimen
Format Journal Article
LanguageChinese
Published China 01.03.2013
Subjects
Adenoviridae - genetics
Animals
Bone Morphogenetic Proteins - genetics
Bone Morphogenetic Proteins - metabolism
Cell Differentiation
Cells, Cultured
Chondrocytes - cytology
Chondrocytes - metabolism
Collagen Type II - metabolism
Female
Fibrocartilage - cytology
Fibrocartilage - metabolism
Flow Cytometry
Genetic Vectors
Male
Mesenchymal Stromal Cells - cytology
Mesenchymal Stromal Cells - metabolism
Rabbits
Reverse Transcriptase Polymerase Chain Reaction
Synovial Membrane - cytology
Tissue Engineering - methods
Transfection
Online AccessGet more information

Cover

More Information
Summary:To investigate the feasibility of rabbit synovial-derived mesenchymal stem cells (SMSCs) differentiating into fibrocartilage cells by the recombinant adenovirus vector mediated by bone morphogenetic protein 2/7 (BMP-2/7) genes in vitro. SMSCs were isolated and purified from 3-month-old New Zealand white rabbits [male or female, weighing (2.1 +/- 0.3) kg]; the morphology was observed; the cells were identified with immunocytological fluorescent staining, flow cytometry, and cell cycles. The adipogenic, osteogenic, and chondrogenic differentiations were detected. The recombinant plasmid of pAdTrack-BMP-2-internal ribosome entry site (IRES)-BMP-7 was constructed and then was used to infect SMSCs. The cell DNA content and the oncogenicity were tested to determine the safety. Then infected SMSCs were cultured in incomplete chondrogenic medium in vitro. Chondrogenic differentiation of infected SMSCs was detected by RT-PCR, immunofluorescent staining, and toluidine blue staining. SMSCs expressed surface markers of s
ISSN:1002-1892
DOI:10.7507/1002-1892.20130079