Progress in co-immobilization of multiple enzymes

• Published in: Shengwu gongcheng xuebao Vol. 31; no. 4; p. 469
• Main Authors: Wang, Jindan, Zhang, Guangya
• Format: Journal Article
• Language: Chinese
• Published: China 01.04.2015
• Subjects: Biocatalysis; Enzymes, Immobilized
• ISSN: 1000-3061
• DOI: 10.13345/j.cjb.140346

Enzyme immobilization is the core technology of biocatalysis. Over the past few decades, enzyme immobilization research mainly focused on single enzyme immobilization. In recent years, multi-enzyme immobilization attracts more and more attention as it could increase the local concentration of reaction and improve the reaction yield. In this review, a summary of the recent progress, together with our research, is presented. Special emphasis is placed on four methods in multi-enzymes co-immobilization, namely, the nonspecific covalent co-immobilization, the nonspecific non-covalent co-immobilization, the non-covalent encapsulation co-immobilized and the site specificity co-immobilized. Finally, some industrial uses of immobilized multi-enzymes were addressed and the application prospect of multi-enzyme immobilization was highlighted.