Comparison between transgenic insect-resistant cotton expressing Cry1Ac protein and its parental variety in rhizospheric fungal diversity

• Published in: Huanjing kexue Vol. 35; no. 11; p. 4329
• Main Authors: Pan, Jian-Gang, Jiao, Hai-Hua, Bai, Zhi-Hui, Qi, Hong-Yan, Ma, An-Zhou, Zhuang, Guo-qiang, Zhang, Hong-xun
• Format: Journal Article
• Language: Chinese
• Published: China 01.11.2014
• Subjects: Animals; Bacterial Proteins - genetics; Endotoxins - genetics; Fungi - classification; Gossypium - genetics; Gossypium - microbiology; Hemolysin Proteins - genetics; Plants, Genetically Modified - microbiology; Rhizosphere
• ISSN: 0250-3301
• DOI: 10.13227/j.hjkx.2014.11.040

The dynamics of rhizospheric fungal diversity and biomass at different sampling stages associated with two transgenic insectresistant cottons expressing Cry1Ac protein and their control varieties were studied under greenhouse conditions, followed by PCR-denaturing gradient gel electrophoresis (PCR-DGGE) and quantitative real-time polymerase chain reaction (Q-PCR), in order to evaluate the ecological security of planting transgenic cotton expressing Cry1Ac protein. The results indicated that the fungal superior bands in rhizosphere of transgenic Bt cotton were similar with that of control cotton at four sampling stages, the more obvious difference in the blurred bands among transgenic Bt cotton, JM20 and SHIYUAN321 was detected. The rhizospheric fungal biomass of transgenic Bt cotton SGK321 was significantly lower than that of its parental control cotton at seedling stage, while the slight decrease in fungal biomass of transgenic Bt cotton XP188 was detected at boll forming stage, the ill-defined decrease, eve