In vitro development and chimeric efficiency of mouse-porcine interspecies chimeric embryos in different culture systems

• Published in: Shengwu gongcheng xuebao Vol. 32; no. 7; p. 975
• Main Authors: Wang, Ying, Ren, Jilong, Song, Yuran, Hai, Tang, Zhou, Qi, Liu, Zhonghua
• Format: Journal Article
• Language: Chinese
• Published: China 25.07.2016
• Subjects: Animals; Blastocyst; Chimera; Culture Media; Embryo Culture Techniques - veterinary; Embryo, Mammalian; Embryonic Development; Induced Pluripotent Stem Cells - cytology; Mice; Swine; porcine; induced pluripotent stem cells; chimeric injection; interspecies chimera; embryo development in vitro
• ISSN: 1000-3061
• DOI: 10.13345/j.cjb.150433

With the advancements of stem cells and regenerative medicine, interspecies chimera has become a hot topic and will pave a new way of providing donor sources in organ transplantation. However, the interspecies chimera is confronted with a number of scientific questions and technical obstacles, including selections of appropriate embryonic stage and appropriate culture medium; those factors will deeply influence the developmental balance between donor cells and receptor embryos. Due to its relatively rapid reproductive cycle and similar organ size to human's, porcine is a very potential donor candidate to study these questions. To compare the development and chimeric efficiency of interspecies embryos, we tested and evaluated three different culture systems, PZM-3 (Porcine zygotic medium), culture medium for iPSCs (N2B27) and 3.5 h of N2B27 before PZM-3 (N2B27(3.5 h)), and two different embryonic stages, 8-cell and blastocyst in mouse-porcine chimeric embryos using parthenogenetically activated porcine embryos