Cytotoxicity of a new type silicone rubber for maxillofacial prosthesis: an in vitro evaluation

This study evaluated the cytotoxicity of a new type silicone rubber for maxillofacial prosthesis, which was developed by the present authors. According to the GB/T16886. 5- 2003, the samples were prepared and tested with cell counting kit-8 (CCK-8) assay, the relative growth rate (RGR) was calculate...

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Published inSheng wu yi xue gong cheng xue za zhi Vol. 31; no. 5; p. 1046
Main Authors Tian, Ai, Chen, Yue, Liao, Jian, Sun, Xu, Teng, Minhua, Liang, Xing
Format Journal Article
LanguageChinese
Published China 01.10.2014
Subjects
Animals
Biocompatible Materials
Cell Line
Humans
Maxillofacial Prosthesis
Mice
Silicone Elastomers - toxicity
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Summary:This study evaluated the cytotoxicity of a new type silicone rubber for maxillofacial prosthesis, which was developed by the present authors. According to the GB/T16886. 5- 2003, the samples were prepared and tested with cell counting kit-8 (CCK-8) assay, the relative growth rate (RGR) was calculated, and morphology of L929 cells were observed by scanning electron microscope and phase contrast microscope. The results showed that RGR of L929 cells were 91.65% (24 h), 87.03% (48 h), 87.30% (72 h), respectively, and the level of cytotoxicity was grade 1. The L929 cells showed typical fusiform shape and their morphology did not changed significantly after 24 h, 48 h and 72 h. These data indicated that the newly-developed silicone rubber material, as a maxillofacial prosthesis material, should be a safe biomaterial.
ISSN:1001-5515