Escherichia coli defects caused by null mutations in dnaK and dnaJ genes

Escherichia coli dnaJ and dnaKdnaJ mutants are defective in cell growth and survival at high temperature. Lack of DnaK and DnaJ proteins results in cell filamentation and leads to the defect in motility. Synthesis of DNA and protein is inhibited at 42 degrees C, especially in double-deletion mutant....

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Bibliographic Details
Published inActa microbiologica Polonica Vol. 46; no. 1; p. 7
Main Authors Paciorek, J, Kardyś, K, Lobacz, B, Wolska, K I
Format Journal Article
LanguageEnglish
Published Poland 1997
Subjects
Bacterial Proteins - biosynthesis
Bacterial Proteins - genetics
Bacterial Proteins - physiology
DNA, Bacterial - biosynthesis
Escherichia coli - genetics
Escherichia coli - growth & development
Escherichia coli - metabolism
Escherichia coli Proteins
Genes, Bacterial - physiology
Heat-Shock Proteins - genetics
Heat-Shock Proteins - physiology
HSP40 Heat-Shock Proteins
HSP70 Heat-Shock Proteins - genetics
HSP70 Heat-Shock Proteins - physiology
Mutation
Temperature
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Summary:Escherichia coli dnaJ and dnaKdnaJ mutants are defective in cell growth and survival at high temperature. Lack of DnaK and DnaJ proteins results in cell filamentation and leads to the defect in motility. Synthesis of DNA and protein is inhibited at 42 degrees C, especially in double-deletion mutant. Degradation of protein was observed in both mutants at high temperature. Complementation of these defects can be achieved by the expression of the wild-type alleles from low-copy number plasmid.
ISSN:0137-1320