Relationship between orthodontics root resorption following experimental tooth movement and the level of dentin sialoph-osphoprotein and dentin sialoprotein in gingival crevicular fluid

• Published in: Hua xi kou qiang yi xue za zhi Vol. 29; no. 3; p. 294
• Main Authors: Zuo, Zhi-Gang, Hu, Min, Jiang, Huan, Tian, Li
• Format: Journal Article
• Language: Chinese
• Published: China 01.06.2011
• Subjects: Animals; Dentin; Extracellular Matrix Proteins; Gingival Crevicular Fluid; Incisor; Male; Orthodontics; Phosphoproteins; Rats; Rats, Wistar; Root Resorption; Sialoglycoproteins; Tooth Movement Techniques
• ISSN: 1000-1182
• DOI: 10.3969/j.issn.1000-1182.2011.03.019

To investigate the relationship of expression of dentin sialoph-osphoprotein (DSPP) and dentin sialoprotein (DSP) in gingival crevicular fluid (GCF) with root resorption following experimental tooth movement in rats. 36 Wistar rats were divided into 3 groups on average randomly: Control group, light force group and heavy force group. The experimental teeth were drawn-off mesially by the force of 0.392 N in light force group and 0.98 N in heavy force group, with both of the maxillary central incisors as the tooth of anchorage. At the 7th day, the gingival crevicular fluid of rats were collected; the histological slices were made, including the experimental tooth and periodontal tissue; the tissues was stained with hematoxylin-eosin (HE) staining and tartrate resistant acid phosphatase (TRAP) staining to observe the histological changes of the root resorption of rats. Then the expression of DSPP and DSP were assayed by using biochemistry techniques of Western blot. Histological observation: There was not root r