In vitro Acaricidal Activity of SerratiaUreilytica Against the Dust Mite TyrophagusPutrescentiae and Identification of Genes Related to Biocontrol

• Published in: Current microbiology Vol. 81; no. 7; p. 199
• Main Authors: Espinosa-Zaragoza, Saúl, Domínguez-Liévano, Alexis, Gómez-Gutiérrez, Jaime Adriel, Wong-Villarreal, Arnoldo, Aguilar-Marcelino, Liliana, Cerqueda-García, Daniel, Rangel-Zaragoza, José Luis, Sanzón-Gómez, Diana, Mireles-Arriaga, Ana Isabel, Sachman-Ruíz, Bernardo
• Format: Journal Article
• Language: English
• Published: New York Springer US 01.07.2024; Springer Nature B.V
• Subjects: Bacteria; Biological control; Biomedical and Life Sciences; Biotechnology; Genes; Genomes; Life Sciences; Microbiology; Nodules; Nucleotides; Phylogeny; Serratia; Stored products
• ISSN: 0343-8651; 1432-0991
• DOI: 10.1007/s00284-024-03728-3

The present study evaluated the acaricidal activity of three Serratia strains isolated from Mimosa pudica nodules in the Lancandon zone Chiapas, Mexico. The analysis of the genomes based on the Average Nucleotide Identity, the phylogenetic relationships allows the isolates to be placed in the Serria ureilytica clade. The size of the genomes of the three strains is 5.4 Mb, with a GC content of 59%. The Serratia UTS2 strain presented the highest mortality with 61.41% against Tyrophagus putrescentiae followed by the Serratia UTS4 strain with 52.66% and Serratia UTS3 with 47.69% at 72 h at a concentration of 1X10 9 cell/mL. In the bioinformatic analysis of the genomes, genes related to the synthesis of chitinases, proteases and cellulases were identified, which have been reported for the biocontrol of mites. It is the first report of S. ureilytica with acaricidal activity, which may be an alternative for the biocontrol of stored products with high fat and protein content.