Genetic diversity of eukaryotic picoplankton of eight lakes in Nanjing

The method of terminal restriction fragment length polymorphism (T-RFLP) was used to study the genetic diversity of eukaryotic picoplankton (0.2-5.0 microm) in the pelagic and littoral zones in 8 lakes with different trophic status in Nanjing. The objectives of this study were to confirm the differe...

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Bibliographic Details
Published inHuanjing kexue Vol. 31; no. 5; p. 1293
Main Authors Zhao, Bi-ying, Chen, Mei-jun, Sun, Ying, Chen, Fei-zhou, Yang, Jia-xin
Format Journal Article
LanguageChinese
Published China 01.05.2010
Subjects
China
Chlorophyll - analysis
Fresh Water - analysis
Genetic Variation
Plankton - genetics
Polymorphism, Restriction Fragment Length
Water Microbiology
China
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Summary:The method of terminal restriction fragment length polymorphism (T-RFLP) was used to study the genetic diversity of eukaryotic picoplankton (0.2-5.0 microm) in the pelagic and littoral zones in 8 lakes with different trophic status in Nanjing. The objectives of this study were to confirm the difference of the genetic diversity of eukaryotic picoplankton among lakes and the main factors affecting this difference. T-RFLP indicated that there were various fingerprints among lakes and zones. The average terminal restriction fragments (T-RFs) in the littoral and pelagic zones were 16.4 and 15.9, respectively. The littoral zone in Lake Nan and the pelagic zone in Lake Mochou had 30 T-RFs and 27 T-RFs, respectively. The T-RFs were the least abundant (10) in the pelagic zone in Lake Baijia with relatively low trophic status. The genetic diversity of eukaryotic picoplankton was higher in the littoral zone than that in the pelagic zone except Lake Pipa and Mochou. The cluster analysis indicated that the similarities of
ISSN:0250-3301