Establishment of a bovine epithelial mammary cell line and its ultrastructural changes when exposed to heat stress

A simple method of trypsin/collagenase I alternative digestion and iterative culture flask adherence to discard fibroblasts for bovine mammary cell culture was established in this study. By immunohistochemistry, flow cytometry, western blot, Electron microscopy analysis, the characteristics of bovin...

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Bibliographic Details
Published inShengwu gongcheng xuebao Vol. 23; no. 3; p. 471
Main Authors Du, Juan, Di, He-Shuang, Wang, Gen-Lin
Format Journal Article
LanguageChinese
Published China 01.05.2007
Subjects
Animals
Apoptosis
Blotting, Western
Caseins - metabolism
Cattle
Cell Line
Cell Nucleus - ultrastructure
Cells, Cultured
Chromatin - ultrastructure
Epithelial Cells - cytology
Epithelial Cells - metabolism
Epithelial Cells - ultrastructure
Female
Flow Cytometry
Hot Temperature
Immunohistochemistry
Mammary Glands, Animal - cytology
Mammary Glands, Animal - metabolism
Microscopy, Electron, Transmission
Mitochondria - ultrastructure
Vimentin - metabolism
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Summary:A simple method of trypsin/collagenase I alternative digestion and iterative culture flask adherence to discard fibroblasts for bovine mammary cell culture was established in this study. By immunohistochemistry, flow cytometry, western blot, Electron microscopy analysis, the characteristics of bovine mammary cells were investigated in vitro. Effect of hyperthermia on the cell ultrastructures was also observed. The results showed that the mammary cells were diploid epithelia with intact 30 pairs chromatins, which could secrete alpha-casein into the medium. After exposed to hyperthermia, the cell condensed chromatin like crescent on the nuclei verges, mitochondria occurred expansion and vacuolization, and apoptotic bodies appeared, which suggested that heat stress could induce apoptosis of the mammary epithelia.
ISSN:1000-3061