Recombinant VLP Vaccines Synthesized in Plant Expression Systems: Current Updates and Prospects

The development and creation of a new generation vaccines based on recombinant proteins that assemble into virus-like particles (VLPs), as well as recombinant proteins in the form of nanoparticles, are promising directions in modern biotechnology. Due to their large size (20–200 nm) and multiplicity...

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Published inMolecular biology (New York) Vol. 58; no. 3; pp. 402 - 418
Main Authors Rozov, S. M., Deineko, E. V.
Format Journal Article
LanguageEnglish
Published Moscow Pleiades Publishing 2024
Springer Nature B.V
Subjects
Antigenic determinants
Biochemistry
Biomedical and Life Sciences
Biosynthesis
Biotechnology
Chloroplasts
Human Genetics
Immune response (cell-mediated)
Immune response (humoral)
Life Sciences
Nanoparticles
Proteins
Reviews
Vaccines
Virus-like particles
recombinant proteins
virus-like particles
transient expression
plant expression systems
stable expression
VLP vaccine
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Summary:The development and creation of a new generation vaccines based on recombinant proteins that assemble into virus-like particles (VLPs), as well as recombinant proteins in the form of nanoparticles, are promising directions in modern biotechnology. Due to their large size (20–200 nm) and multiplicity of viral antigenic determinants on the surface, VLPs can stimulate strong humoral and cellular immune responses. The main types of VLPs, as well as the features and disadvantages of the main expression systems used for their biosynthesis, are considered in this review. The main focus was on plant expression systems that ensure the biosynthesis of a target recombinant protein from a DNA matrix integrated into the nuclear or chloroplast genomes of a plant (stable expression) or from a matrix for temporary production of the target product (transient expression). Various approaches for increasing the yield of VLP-forming recombinant proteins, including fusion with a transit peptide that directed the protein into the chloroplast, were discussed. The possibility of accumulation of recombinant proteins expressed in plants and intended for creation of VLP-vaccines in another type of nanoparticle, protein bodies, using specific signal sequences was also considered.
ISSN:0026-8933
1608-3245
DOI:10.1134/S0026893324700043