Bacterial diversity in a sequencing batch reactor treating nitrogen-containing aromatic wastewater

• Published in: Huanjing kexue Vol. 29; no. 9; p. 2564
• Main Authors: Liu, Xing-yu, Wang, Bao-jun, Zhao, Ke-xin, Liu, Lei, Jiang, Cheng-yin, Liu, Shuang-jiang
• Format: Journal Article
• Language: Chinese
• Published: China 01.09.2008
• Subjects: Aniline Compounds - chemistry; Aniline Compounds - metabolism; Bacteria - classification; Bacteria - growth & development; Bacteria - metabolism; Biodegradation, Environmental; Biodiversity; Bioreactors - microbiology; Hydrocarbons, Aromatic - chemistry; Hydrocarbons, Aromatic - metabolism; Molecular Structure; Nitrobenzenes - chemistry; Nitrobenzenes - metabolism; Nitrogen - chemistry; Nitrogen - metabolism; Nitrophenols - chemistry; Nitrophenols - metabolism; Waste Disposal, Fluid - methods
• ISSN: 0250-3301

For understanding the mechanism of biodegradation in a SBR reactor treating nitrogen-containing aromatic wastewater, the reactor sludge samples were used for PCR-DGGE analysis. Meanwhile, 90 strains were isolated from the sludge sample after the start-up stage, 36 strains can degrade nitrogen-containing aromatic compounds. 5 strains nitrogen-containing aromatic degraders were further performed aromatic ring cleavage dioxygenase analysis. Result showed that bacterial diversity in the reactor were changed during the start-up stage, Acidobacteria (SBR1, SBR7), Actinobacteria (SBR4) and beta-Proteobacteria (SBR6) may play important role in biodegradation of nitrogen-containing aromatic compounds, among the isolates. Different bacterial diversity research method all have bias, and reflected different part of the bacterial structure. Among the isolates, Actinobacteria were the majority, by analysis the ring cleavage enzyme activity, further understood how the four nitrogen-containing aromatic compound were degraded