Molecular characterization of a genotype II virulent Newcastle disease virus isolate from chicken

Newcastle disease virus (NDV) field strain SQZ04 was isolated from a broiler flock with typical symptoms and lesions, and cloned by plaque-purification three times. NDV SQZ04 was determined as a virulent strain with MDT of 50.5h and 51.2h, ICPI of 2.0 and 1.92, IVPI of 2.8 and 2.68 respectively befo...

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Bibliographic Details
Published inWei sheng wu hsüeh pao Vol. 46; no. 6; p. 912
Main Authors Qin, Zhuo-ming, Ma, Bao-chen, Jia, Qiang, Ouyang, Wen-jun, Cui, Zhi-zhong, Xu, Huai-ying, Yuan, Xiao-yuan
Format Journal Article
LanguageChinese
Published China 01.12.2006
Subjects
Animals
Chickens - virology
Genotype
HN Protein - genetics
Newcastle disease virus - classification
Newcastle disease virus - genetics
Phylogeny
Viral Fusion Proteins - genetics
Virulence
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Summary:Newcastle disease virus (NDV) field strain SQZ04 was isolated from a broiler flock with typical symptoms and lesions, and cloned by plaque-purification three times. NDV SQZ04 was determined as a virulent strain with MDT of 50.5h and 51.2h, ICPI of 2.0 and 1.92, IVPI of 2.8 and 2.68 respectively before and after plaque-purification. Analysis of F gene indicated that SQZO4 was determined as a virulent gene type II , and its protein amino acid sequence has homologies of 99.3% , 98.7% and 96.9% with published gene type II vaccine strains LaSota, B1, virulent strain Taxas48,much higher than homologies of 88.3% - 88.6% or 91.3% - 92.1% with published gene types VI and IX. This is the first virulent field strain of gene type UI reported in China. Further more, the amino acid sequence 111 GGRQGRL117 in the F protein cleavage site in SQZ04 strain is identical to lentogenic strains of NDV, such as vaccine strains LaSota, B1. This is the first report that virulent NDV could have lentogenic amino acid sequence in the cle
ISSN:0001-6209