Specific identification of (R)-3-hydroxyacyl-ACP: CoA transacylase gene from Pseudomonas and Burkholderia strains by polymerase chain reaction

Polyhydroxyalkanoates (PHA) were biodegradable thermoplastics. Due to their broad applications, direct biosynthesis of PHA from inexpensive substrates, such as carbohydrates, is actively pursued. It has been recently revealed that (R)-3-hydroxyacyl-ACP: CoA transacylase (PhaG) played an important ro...

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Bibliographic Details
Published inShengwu gongcheng xuebao Vol. 21; no. 1; p. 19
Main Authors Zheng, Zhong, Chen, Jin-Chun, Tian, Hong-Lei, Bei, Feng-Feng, Chen, Guo-Qiang
Format Journal Article
LanguageEnglish
Published China 01.01.2005
Subjects
Acyltransferases - genetics
Acyltransferases - metabolism
Amino Acid Sequence
Burkholderia - enzymology
Burkholderia - genetics
Genes, Bacterial
Molecular Sequence Data
Polyhydroxyalkanoates - biosynthesis
Polymerase Chain Reaction
Pseudomonas - enzymology
Pseudomonas - genetics
Sequence Alignment
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Summary:Polyhydroxyalkanoates (PHA) were biodegradable thermoplastics. Due to their broad applications, direct biosynthesis of PHA from inexpensive substrates, such as carbohydrates, is actively pursued. It has been recently revealed that (R)-3-hydroxyacyl-ACP: CoA transacylase (PhaG) played an important role in this pathway. In this study, a polymerase chain reaction (PCR) protocol was developed for the rapid and specific identification of phaG gene from various bacteria. Using the PCR strategy, the complete open reading frames of two phaG genes from Pseudomonas stutzeri 1317 and Pseudomonas nitroreducens 0802 were cloned from the genomic DNA and functionally expressed in Pseudomonas putida PHAGN-21. Furthermore, this strategy was successful applied in non-Pseudomonas strains, such as Burkholderia. These results suggest that PhaG-mediated pathway of medium-chain-length polyhydroxyalkanoates was widespread among bacteria.
ISSN:1000-3061