Isolation and characterization of a benzothiophene-desulfurizing bacterium

A benzothiophene-desulfurizing strain was isolated and identified as Gordonia sp. C-6, which could degrade benzothiophene (BT) in a way analogous to the 4S pathway. It was found strain C-6 had the ability to grow in KT-BT medium whose sole source of sulfur was BT or its derivatives, whereas it could...

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Bibliographic Details
Published inHuanjing kexue Vol. 27; no. 6; p. 1181
Main Authors Li, Guo-qiang, Lee, Kyongho, Ma, Ting, Li, Shan-shan, Li, Hong, Liang, Feng-lai, Liu, Ru-lin
Format Journal Article
LanguageChinese
Published China 01.06.2006
Subjects
Gordonia Bacterium - classification
Gordonia Bacterium - isolation & purification
Gordonia Bacterium - metabolism
Phylogeny
RNA, Ribosomal, 16S - genetics
Soil Microbiology
Sulfur - metabolism
Thiophenes - metabolism
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Summary:A benzothiophene-desulfurizing strain was isolated and identified as Gordonia sp. C-6, which could degrade benzothiophene (BT) in a way analogous to the 4S pathway. It was found strain C-6 had the ability to grow in KT-BT medium whose sole source of sulfur was BT or its derivatives, whereas it couldn't grow well in a medium with DBT and its derivatives as the sole source of sulfur, the desulfurized product was identified as 2-(2'-hydroxyphenyl) ethan-1-al or its isomer benzofuran by GC-MS analysis. Desulfurization activity of strain C-6 was investigated. About 0.15mmol/L(50%) BT was desulfurized by strain C-6 after it was incubated in KT-BT medium at 30 degrees C for 48h, the residual BT has volatilized during aerobic fermentation. The quantitative detection of the benzothiophene-desulfurizing products was established with o-hydroxyphenylacetic acid as standard compound, the standard curve was fitted by Matlab.
ISSN:0250-3301