Effects of lysosomal Fe2+ on mitochondria apoptosis and muscle tenderness in postmortem Tibetan sheep meat

• Published in: International journal of food science & technology Vol. 59; no. 9; pp. 6277 - 6288
• Main Authors: Wang, Jingyu, Han, Jinhua, Zhao, Ruina, Yu, Qunli, Chen, Cheng, Guo, Zhaobin, Wen, Jingtao, Fan, Guozhong, Shi, Xixiong
• Format: Journal Article
• Language: English
• Published: Oxford Wiley Subscription Services, Inc 01.09.2024
• Subjects: Apoptosis; Caspase; Caspase‐3; Cathepsins; Correlation analysis; Cytochrome c; Deferoxamine; Distilled water; Iron; lysosome; Meat; Membrane permeability; Membrane potential; Membranes; mitochondrial damage; Mitochondrial permeability transition pore; Muscles; Principal components analysis; Reactive oxygen species; Shear forces; Sheep; Stability; tenderisation; Tibetan sheep meat
• ISSN: 0950-5423; 1365-2621
• DOI: 10.1111/ijfs.17366

Summary In this work, deferoxamine (DFO) was injected into Tibetan sheep meat (distilled water as control) to reveal the effects of lysosomal Fe2+ on mitochondrial apoptosis and muscle tenderness. The reactive oxygen species (ROS) content, lysosomal membrane stability, cathepsins activity, mitochondrial membrane permeability transition pore (MPTP), mitochondrial membrane potential (MMP), cytochrome c (Cyt‐c) reduction level, caspase activity, myofibril fragmentation index (MFI) and shear force of Oula Tibetan sheep meat were measured at different ageing times. The results indicated that the ROS content, cathepsins activity, MPTP opening degree, lysosomal membrane stability, caspase‐3/9 activity and MFI of Tibetan sheep meat were significantly decreased after DFO treatment, whilst MMP, shear force and Cyt‐c reduction levels were noticeably increased. Furthermore, correlation analysis and principal component analysis showed that mitochondrial damage was positively correlated with meat tenderness. In sum, the study suggests that lysosomal Fe2+ may promote mitochondrial apoptosis and improve the tenderness of Tibetan sheep meat. In this work, deferoxamine (DFO) was injected into Tibetan sheep meat (distilled water as control) to reveal the effects of lysosomal Fe2+ on mitochondrial apoptosis and muscle tenderness. We found that lysosomal Fe2+ can promote mitochondrial apoptosis and improve the tenderness of Tibetan sheep meat.