Use of Commercial Enzymes to Boost On-Site Enzyme Efficiency

• Published in: Renewable Biofuels pp. 87 - 104
• Main Authors: Rana, Diwakar, Rana, Vandana
• Format: Book Chapter
• Language: English
• Published: Switzerland Springer International Publishing AG 2017; Springer International Publishing
• Series: SpringerBriefs in Applied Sciences and Technology
• Subjects: Alternative & renewable energy sources & technology; Cellulose Conversion; Commercial Enzyme; Corn Steep Liquor; Corn Stover; Enzyme Cocktail; Industrial chemistry; Power generation & distribution
• ISBN: 3319473786; 9783319473789
• ISSN: 2191-530X; 2191-5318
• DOI: 10.1007/978-3-319-47379-6_4

In-house production of cellulases from filamentous fungi is widely used, but their hydrolytic efficiency compared to commercial enzymes is limited. We studied the effect of supplementing in-house cellulases produced by Trichoderma reesei RUT-C30 and a novel strain Aspergillus saccharolyticus with different types of commercial enzymes for the efficient hydrolysis of wet-exploded loblolly pine. Cellic®Ctec 2, Cellic®Htec2, and Novozym 188 were used as the commercial base enzymes for supplementing the in-house produced enzymes. Compared to non-supplemented in-house enzyme preparation, commercial enzymes (Cellic®Ctec2) added in the same amount as FPU and CBU resulted in 68 % higher glucose yield using wet-exploded loblolly pine (WELP) at a 20 % DM concentration. Highest saccharification yield was achieved by supplementation of the in-house produced cellulases with Cellic®Htec2 compared to Cellic®Ctec2 and Novozym 188. Optimal glucose, xylose, and mannose yields, 85 %, 92 %, and 86 %, respectively, were achieved by using in-house enzymes (15 FPU/g cellulose) supplemented with commercial hemicellulase (7.5 FPU/g cellulose). These results showed that supplementing in-house enzymes with commercial enzymes can be advantageous and work for lowering the overall cost of enzymes in a biorefinery.