Enzyme Production from Trichoderma reesei and Aspergillus Strain

• Published in: Renewable Biofuels pp. 69 - 86
• Main Authors: Rana, Diwakar, Rana, Vandana
• Format: Book Chapter
• Language: English
• Published: Switzerland Springer International Publishing AG 2017; Springer International Publishing
• Series: SpringerBriefs in Applied Sciences and Technology
• Subjects: Alternative & renewable energy sources & technology; Corn Stover; Glucose Yield; Industrial chemistry; Lignocellulosic Biomass; Power generation & distribution; Sugar Yield; Xylanase Activity
• ISBN: 3319473786; 9783319473789
• ISSN: 2191-530X; 2191-5318
• DOI: 10.1007/978-3-319-47379-6_3

Cellulase production by two filamentous fungi Trichoderma reesei RUT-C30 and novel fungal strain, Aspergillus saccharolyticus, on pretreated corn stover was investigated. Cellulase production was followed by the hydrolysis of wet-exploded corn stover (WECS) and wet-exploded loblolly pine (WELP) by on-site produced enzyme cocktail containing cellulase from T. reesei RUT-C30 and β-glucosidase from A. saccharolyticus. The sugar yields by using the on-site enzyme cocktail were compared with the commercial enzyme preparations, Celluclast 1.5 L and Novozym 188 at two substrate concentrations, 5 and 10 % (w/w), and enzyme loading at 5 and 15 FPU/g glucan for WECS and WELP. Highest sugar yields were obtained at 5 % (w/w) substrate concentration and 15 FPU/g glucan for both feedstocks, WECS and WELP. Glucose yields of 81 and 88 % were obtained from on-site and commercial enzymes, respectively, from WECS, and 55 and 58 % were achieved from on-site and commercial enzymes, respectively, from WELP.