The potential role of long non-coding RNA Dnm3os in the activation of cardiac fibroblasts

• Published in: Sheng wu yi xue gong cheng xue za zhi Vol. 38; no. 3; p. 574
• Main Authors: Kong, Qihang, Zhou, Junteng, Tian, Geer, Quan, Yue, Wu, Wenchao, Liu, Xiaojing
• Format: Journal Article
• Language: Chinese
• Published: China Sichuan Society for Biomedical Engineering 25.06.2021
• Subjects: Aorta; Collagen; Contraction; Fibroblasts; Fibrosis; Growth factors; Heart; Humans; Lung diseases; Myocardium - pathology; Non-coding RNA; Phenotypes; Polymerase chain reaction; RNA, Long Noncoding; Signal processing; Signal Transduction; siRNA; Smad2 protein; Thorax; Transforming Growth Factor beta1; Transforming growth factor-b1; transforming growth factor-β1/Smad2/3; cardiac fibrosis; long non-coding RNA Dnm3os; cardiac fibroblast activation
• ISSN: 1001-5515
• DOI: 10.7507/1001-5515.202102021

Long non-coding RNA (lncRNA) Dnm3os plays a critical role in peritendinous fibrosis and pulmonary fibrosis, but its role in the process of cardiac fibrosis is still unclear. Therefore, we carried out study by using the myocardial fibrotic tissues obtained by thoracic aortic constriction (TAC) in an early study of our group, and the cardiac fibroblast activation model induced by transforming growth factor-β1 (TGF-β1). Quantitative real-time polymerase chain reaction (RT-qPCR), Western blot, and collagen gel contraction test were used to identify the changes of activation phenotype and the expression of Dnm3os in cardiac fibroblasts. Small interfering RNA was used to silence Dnm3os to explore its role in the activation of cardiac fibroblasts. The results showed that the expression of Dnm3os was increased significantly in myocardial fibrotic tissues and in the activated cardiac fibroblasts. And the activation of cardiac fibroblasts could be alleviated by Dnm3os silencing. Furthermore, the TGF-β1/Smad2/3 pathway