Evaluation of a two step AFB cold staining method and simplified concentration technique for diagnosis of pulmonary tuberculosis

Ziehl Neelsen (Z-N) stain is the commonly used procedure for demonstration for AFB. A Simple cold stain technique and Simplified concentration technique were compared with the conventional methods on 1484 sputum samples over a period of 3 months. A positivity rate of 9.3% was observed on direct micr...

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Bibliographic Details
Published inJournal of communicable diseases Vol. 34; no. 4; p. 276
Main Author Kochhar, Abhilasha
Format Journal Article
LanguageEnglish
Published India 01.12.2002
Subjects
Evaluation Studies as Topic
Humans
India
Microscopy - methods
Sputum - microbiology
Staining and Labeling - methods
Tuberculosis, Pulmonary - diagnosis
India
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Summary:Ziehl Neelsen (Z-N) stain is the commonly used procedure for demonstration for AFB. A Simple cold stain technique and Simplified concentration technique were compared with the conventional methods on 1484 sputum samples over a period of 3 months. A positivity rate of 9.3% was observed on direct microscopy with an increase in the yield by 30% after concentration. It was observed that the simple cold staining and simplified concentration are viable alternatives for demonstration of mycobacteria in busy clinical laboratories.
ISSN:0019-5138