Langmuir films from antibodies based on amphiphilic polyelectrolytes

A technique for forming Langmuir films from antibodies based on an amphiphilic polyelectrolyte was developed. The physicochemical and immunochemical properties of the Langmuir films obtained were studied. The interaction of HBsAg with the films was found to be described by a model with one binding s...

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Bibliographic Details
Published inMembrane & cell biology Vol. 10; no. 6; p. 689
Main Authors Babitskaya, J I, Budashov, I A, Chernov, S F, Kurochkin, I N, Doroshenko, N V, Zubov, S V
Format Journal Article
LanguageEnglish
Published Switzerland 1997
Subjects
Animals
Electrolytes - chemistry
Electrolytes - metabolism
Goats
Hepatitis B Surface Antigens - chemistry
Hepatitis B Surface Antigens - immunology
Hydrogen-Ion Concentration
Immunoglobulin Fab Fragments - chemistry
Immunoglobulin Fab Fragments - metabolism
Membranes, Artificial
Methylmethacrylate
Methylmethacrylates - chemistry
Osmolar Concentration
Polymers - chemistry
Polymers - metabolism
Surface Properties
Surface-Active Agents - chemistry
Surface-Active Agents - metabolism
Thermodynamics
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Summary:A technique for forming Langmuir films from antibodies based on an amphiphilic polyelectrolyte was developed. The physicochemical and immunochemical properties of the Langmuir films obtained were studied. The interaction of HBsAg with the films was found to be described by a model with one binding site, whereas that of HBsAg with antibodies adsorbed on a polystyrene plate, by a model with a positive cooperativity. The use of the novel Langmuir films from antibodies increases the sensitivity of the immunoenzyme assay.
ISSN:1023-6597