C-myb proto-oncogene: evidence for intermolecular recombination of coding sequences

We have characterized a novel chicken c-myb exon whose sequences are specifically expressed in thymic cells. In situ hybridization experiments indicate that this thymus-specific coding exon is localized on a small chromosome, distinct from the large acrocentric chromosome 3 on which we recently mapp...

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Bibliographic Details
Published inOncogene Vol. 6; no. 4; p. 505
Main Authors Vellard, M, Soret, J, Viegas-Pequignot, E, Galibert, F, Nguyen, V C, Dutrillaux, B, Perbal, B
Format Journal Article
LanguageEnglish
Published England 01.04.1991
Subjects
Animals
Base Sequence
Blotting, Southern
Chickens - genetics
Chromosome Mapping
Chromosomes, Human, Pair 17
Drosophila - genetics
Exons
Humans
Hybrid Cells
Mice
Molecular Sequence Data
Nucleic Acid Hybridization
Proto-Oncogene Proteins - genetics
Proto-Oncogene Proteins c-myb
Recombination, Genetic
RNA, Messenger - analysis
Sequence Homology, Nucleic Acid
Thymus Gland - metabolism
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Summary:We have characterized a novel chicken c-myb exon whose sequences are specifically expressed in thymic cells. In situ hybridization experiments indicate that this thymus-specific coding exon is localized on a small chromosome, distinct from the large acrocentric chromosome 3 on which we recently mapped the bulk of 15 exons, common to the c-myb mRNA species expressed in hematopoietic cells of both B and T lineages. These observations indicate that intermolecular recombination is required for the tissue-specific expression of the c-myb proto-oncogene. We also show that these thymus-specific sequences are conserved in human DNA and lie on chromosome 17q25, whereas the human c-myb locus is localized on chromosome 6q22-23. Sequencing data obtained from genomic DNA and PCR analyses performed with c-myb mRNA species expressed in chicken thymic cells strongly suggest that a repeated decameric sequence plays a key role in the recombination process.
ISSN:0950-9232