In vivo and in vitro effect of the Haemophilus influenzae lipopolysaccharide on ciliated respiratory epithelium

The lipopolysaccharide of Haemophilus influenzae is presumed to have a toxic effect on the tracheal epithelium, and then induce a bronchial obstruction. This activity of LPS was studied in vitro on the ciliary beat using a photo-oscillographic apparatus, and in vivo on the rabbit trachea. Neither mo...

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Bibliographic Details
Published inJournal of hygiene, epidemiology, microbiology, and immunology Vol. 26; no. 2; p. 112
Main Authors Raichvarg, D, Siou, G, Dubreuil, A, Bonnaire, Y, Brossard, C, Boudène, C
Format Journal Article
LanguageEnglish
Published Czech Republic 1982
Subjects
Animals
Cilia - physiology
Epithelium - physiology
Haemophilus influenzae - analysis
Lipopolysaccharides - pharmacology
Movement - drug effects
Rabbits
Rats
Trachea - drug effects
Trachea - physiology
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Summary:The lipopolysaccharide of Haemophilus influenzae is presumed to have a toxic effect on the tracheal epithelium, and then induce a bronchial obstruction. This activity of LPS was studied in vitro on the ciliary beat using a photo-oscillographic apparatus, and in vivo on the rabbit trachea. Neither modification of ciliary beat frequency, nor epithelial damage in the rabbit trachea was observed after a single administration of LPS. In contrast, histopathologic changes were observed in vivo when the intratracheal administration of H. influenzae LPS was followed 24 h later by an intravenous injection of the same LPS. These experimental models seem thus to implicate a Shwartzman type cellular necrosis in the trachea in vivo in the absence of a direct toxic effect of endotoxin itself on trachea in vivo or in vitro.
ISSN:0022-1732