Vaccinia core transcription prevents in vitro depolymerization of polyribosomes in rabbit reticulocyte lysate

When a rabbit reticulocyte lysate is incubated in the presence of vaccinia cores, protein synthesis is impaired at the level of the initiation step and the polyribosomes are depolymerized. However, when the same system is coupled with virus transcription: a) protein synthesis is restored, b) the ini...

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Bibliographic Details
Published inBrazilian journal of medical and biological research Vol. 20; no. 6; pp. 763 - 766
Main Authors Damaso, CRA, Moussatche, N
Format Journal Article
LanguageEnglish
Published Brazil 1987
Subjects
Animals
In Vitro Techniques
Polyribosomes - metabolism
Protein Biosynthesis
Protein Synthesis Inhibitors - pharmacology
Rabbits
Reticulocytes - metabolism
Transcription, Genetic
vaccinia virus
Vaccinia virus - genetics
Vaccinia virus - physiology
Viral Proteins - metabolism
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Summary:When a rabbit reticulocyte lysate is incubated in the presence of vaccinia cores, protein synthesis is impaired at the level of the initiation step and the polyribosomes are depolymerized. However, when the same system is coupled with virus transcription: a) protein synthesis is restored, b) the initiation step is not inhibited, and c) the polyribosomes are not disaggregated. A viral factor activated in the absence of virus transcription and not activated when RNA synthesis occurs may be involved in the early mechanism of protein synthesis inhibition by vaccinia virus.
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ISSN:0100-879X