Rheumatoid adherent synovial cells produce B cell differentiation factor activity neutralizable by antibody to B cell stimulatory factor-2/interleukin 6

B cell differentiation factor (BCDF) activity assayed on SKW6-CL4 cells was found in adherent synovial cell (ASC) conditioned medium. ASC of patients with RA (n = 8) produced significantly larger amounts of BCDF than those of patients with osteoarthritis (n = 5) (219.0 +/- 212.1 vs 25.5 +/- 12.4 uni...

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Published inJournal of rheumatology Vol. 15; no. 11; p. 1616
Main Authors Harigai, M, Kitani, A, Hara, M, Hirose, T, Norioka, K, Suzuki, K, Suzuki, M, Kawagoe, M, Shinmei, M, Nakamura, H
Format Journal Article
LanguageEnglish
Published Canada 01.11.1988
Subjects
Aged
Antibodies - immunology
Arthritis, Rheumatoid - metabolism
B-Lymphocytes - immunology
B-Lymphocytes - metabolism
Cell Line, Transformed
Clone Cells - metabolism
Female
Humans
Interleukin-4
Interleukin-6
Interleukins - biosynthesis
Interleukins - immunology
Interleukins - metabolism
Macrophages - immunology
Macrophages - metabolism
Male
Middle Aged
Neutralization Tests
Osteoarthritis - metabolism
Synovial Fluid - immunology
Synovial Fluid - metabolism
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Summary:B cell differentiation factor (BCDF) activity assayed on SKW6-CL4 cells was found in adherent synovial cell (ASC) conditioned medium. ASC of patients with RA (n = 8) produced significantly larger amounts of BCDF than those of patients with osteoarthritis (n = 5) (219.0 +/- 212.1 vs 25.5 +/- 12.4 units/microgram.DNA, p less than 0.01). ASC clones, established by the limiting dilution technique, also produced this factor. Experiments with several neutralizing antibodies revealed that this BCDF is inhibited by anti-B cell stimulatory factor-2/interleukin 6 antibody (anti-BSF-2/IL-6 ab) up to 90%, but not by antiinterleukin 1, antiinterleukin 2 or antiinterferon-r antibodies. Our data suggest that ASC could participate in the B cell differentiation process in joint space by producing a molecule which has a similar active site to BSF-2/IL-6.
ISSN:0315-162X