A rapid and highly predictive in vitro assay for non-steroidal anti-inflammatory agents

The inhibition of the production of malonyldialdehyde (MDA) in guinea-pig lung homogenates, incubated in the presence of 50 microM arachidonic acid and 1.4 mM adrenaline, has been exploited as a simple and reliable assay to test in vitro non-steroidal anti-inflammatory agents (NSAIA). The inhibitory...

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Bibliographic Details
Published inInternational journal on tissue reactions Vol. 10; no. 2; p. 79
Main Authors Luzzani, F, Ventura, P, Zuccari, G, Clavenna, G
Format Journal Article
LanguageEnglish
Published Switzerland 1988
Subjects
Animals
Anti-Inflammatory Agents, Non-Steroidal - analysis
Anti-Inflammatory Agents, Non-Steroidal - pharmacology
Chemistry Techniques, Analytical - methods
Female
Guinea Pigs
In Vitro Techniques
Male
Malondialdehyde - analysis
Malondialdehyde - antagonists & inhibitors
Osmolar Concentration
Prostaglandin-Endoperoxide Synthases - metabolism
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Summary:The inhibition of the production of malonyldialdehyde (MDA) in guinea-pig lung homogenates, incubated in the presence of 50 microM arachidonic acid and 1.4 mM adrenaline, has been exploited as a simple and reliable assay to test in vitro non-steroidal anti-inflammatory agents (NSAIA). The inhibitory potencies of a series of reference NSAIA, which correlated fairly well with in vivo anti-inflammatory activity as determined by carrageenin oedema, are herewith reported. The specificity of the assay was also evaluated by testing up to forty miscellaneous drugs: none of these significantly reduced the MDA production.
ISSN:0250-0868