Quality control in flow cytometry for diagnostic pathology. I, Cell surface phenotyping and general laboratory procedures

Flow cytometric analyses have become commonplace in the clinical laboratory for determining cell lineage and for quantitation of cells bearing a given phenotype. Because these assays are being conducted to support diagnoses or assist in determining therapy, it is crucial to ensure that these tests a...

Full description

Saved in:
Bibliographic Details
Published inAmerican journal of clinical pathology Vol. 93; no. 4; pp. S27 - S37
Main Authors MCCOY, J. P, CAREY, J. L, KRAUSE, J. R
Format Journal Article
LanguageEnglish
Published Chicago, IL American Society of Clinical Pathologists 01.04.1990
Subjects
Biological and medical sciences
Cell Separation
Data Interpretation, Statistical
Flow Cytometry - instrumentation
Flow Cytometry - standards
Flow Cytometry - statistics & numerical data
Humans
Investigative techniques, diagnostic techniques (general aspects)
Leukocyte Count - instrumentation
Medical sciences
Pathology, Clinical - standards
Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques
Phenotype
Quality Control
Specimen Handling
Staining and Labeling
Human
Flow cytometry
Exploration
Quality control
Online AccessGet full text

Cover

More Information
Summary:Flow cytometric analyses have become commonplace in the clinical laboratory for determining cell lineage and for quantitation of cells bearing a given phenotype. Because these assays are being conducted to support diagnoses or assist in determining therapy, it is crucial to ensure that these tests are highly accurate and reproducible within a laboratory and among laboratories involved in similar endeavors. This quality assurance has been slow evolving in clinical flow cytometry for a variety of reasons: the exquisite sensitivity and delicacy of the instrumentation that recognize previously undetectable variations in staining; the constant improvement of the hardware and software; the rapid development of new techniques and reagents of clinical interest; and the failure of any existing specialty or subspecialty to encompass all aspects of flow cytometry. This article provides an overview of quality assurance necessary for the flow cytometric analysis of cell surface markers. Practical experience, published studies, and suggested guidelines from accreditation agencies have been combined to develop the text.
Bibliography:ObjectType-Article-2
SourceType-Scholarly Journals-1
ObjectType-Feature-3
content type line 23
ObjectType-Review-1
ISSN:0002-9173
1943-7722