Detection of antibodies against Opisthorchis viverrini in patients before and after treatment with praziquantel

Levels of antibody in sera of 78 patients with opisthorchiasis, 30 patients with other liver diseases, 10 patients with schistosomiasis and 30 healthy individuals were compared using three serodiagnostic tests, namely indirect haemagglutination (IHA), enzyme-linked immunosorbent assay (ELISA) and le...

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Published inSoutheast Asian journal of tropical medicine and public health Vol. 19; no. 1; p. 101
Main Authors Thammapalerd, N, Tharavanij, S, Nacapunchai, D, Bunnag, D, Radomyos, P, Prasertsiriroj, V
Format Journal Article
LanguageEnglish
Published Thailand 01.03.1988
Subjects
Animals
Antibodies, Helminth - analysis
Concanavalin A
Enzyme-Linked Immunosorbent Assay
Hemagglutination Tests
Humans
Liver Diseases - immunology
Opisthorchiasis - diagnosis
Opisthorchiasis - drug therapy
Opisthorchis - immunology
Praziquantel - therapeutic use
Schistosomiasis japonica - immunology
Sensitivity and Specificity
Serologic Tests - methods
Thailand
Thailand
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Summary:Levels of antibody in sera of 78 patients with opisthorchiasis, 30 patients with other liver diseases, 10 patients with schistosomiasis and 30 healthy individuals were compared using three serodiagnostic tests, namely indirect haemagglutination (IHA), enzyme-linked immunosorbent assay (ELISA) and lectin immuno test (LIT). The geometric mean reciprocal titer in sera of opisthorchiasis patients was significantly higher than patients with other diseases, patients with schistosomiasis and healthy individuals (p less than 0.00001). After treatment with praziquantel, the antibody titers were decreased and became lowest 120 days after treatment. A statistically significant decrease from the pre-treatment sample was observed only at 120 days after infection and not earlier and only with ELISA (p = 0.03) and not with IHA and LIT (p greater than 0.05). Even with ELISA, significant decrease in antibody titer was apparent only when the pre-treatment sera had high enough antibody titer. ELISA was therefore better than the other two tests for the assessment of cure provided that the titer of pre-treatment sera was high.
ISSN:0125-1562