Study of in vitro drug sensitivity on a newly established cell line from a primary bronchial epidermoid carcinoma of human origin (NSCLCN6)

We studied a cell line established from a primary non-small-cell lung cancer (non-SCLC) of human origin and characterized by midly differentiated epidermoid carcinoma, a human karyotype and keratin expression. Doubling time was about 48 h in vitro and 12 days when transplanted into nude mice. In vit...

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Published inAnticancer research Vol. 11; no. 6; p. 2239
Main Authors Roussakis, C, Gratas, C, Audouin, A F, Le Boterff, J, Dabouis, C, Andre, M J, Moyon, E, Vo, N H, Pradal, G, Verbist, J F
Format Journal Article
LanguageEnglish
Published Greece 01.11.1991
Subjects
Aged
Animals
Carcinoma, Squamous Cell - drug therapy
Carcinoma, Squamous Cell - genetics
Carcinoma, Squamous Cell - pathology
Drug Screening Assays, Antitumor
Female
Humans
Karyotyping
Lung Neoplasms - drug therapy
Lung Neoplasms - genetics
Lung Neoplasms - pathology
Male
Mice
Mice, Nude
Tumor Cells, Cultured
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Summary:We studied a cell line established from a primary non-small-cell lung cancer (non-SCLC) of human origin and characterized by midly differentiated epidermoid carcinoma, a human karyotype and keratin expression. Doubling time was about 48 h in vitro and 12 days when transplanted into nude mice. In vitro, this cell line was mainly sensitive to dactinomycin and mitotic poisons such as Vinca alkaloids. Most chemotherapeutic drugs proved ineffective. Our findings are comparable to previous results in patients who showed 30% objective response and less than 5% complete response regardless of the therapeutic associations used against non-SCLC. Our line would also seem to provide a good model for studying new potentially antitumor substances.
ISSN:0250-7005