Activity of liposomal interleukin-2 in vitro

Preclinical in vitro assessment of highly purified natural human interleukin-2 (IL-2) packed in egg lecithin liposomes was performed in short- and long-term T-cell cloning and propagation systems, and in experiments testing induction of lymphokine-activated killer (LAK) cells. Liposomal IL-2 (lip-IL...

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Bibliographic Details
Published inMolecular biotherapy Vol. 4; no. 1; p. 24
Main Authors Adibzadeh, M, Weder, H G, Rehbein, A, Schwuléra, U, Obermeier, J, Pawelec, G
Format Journal Article
LanguageEnglish
Published United States 01.03.1992
Subjects
Humans
In Vitro Techniques
Interleukin-2 - administration & dosage
Killer Cells, Lymphokine-Activated - immunology
Liposomes
Lymphocyte Activation
T-Lymphocytes - immunology
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Summary:Preclinical in vitro assessment of highly purified natural human interleukin-2 (IL-2) packed in egg lecithin liposomes was performed in short- and long-term T-cell cloning and propagation systems, and in experiments testing induction of lymphokine-activated killer (LAK) cells. Liposomal IL-2 (lip-IL-2) was essentially as active as free natural or recombinant IL-2 for cloning and culture of both helper and cytotoxic alloreactive T cells. However, lip-IL-2 was found to be markedly inferior to free natural or recombinant IL-2 for the induction of LAK cells from normal donors. Nevertheless, lip-IL-2 was able to maintain LAK cytotoxicity of populations preactivated with free IL-2. These results suggest that lip-IL-2 can interact with activated T cells and LAK cells in the same way as free IL-2, but that it is much less efficient at activating LAK-cell precursors.
ISSN:0952-8172