Neurite outgrowth of neuroblastoma cells induced by proteins covalently coupled to glass coverslips

A method using proteins covalently coupled to glass surfaces has been applied to studies on the differentiation of neuroblasts. Neuroblastoma cells from clones N 18 and NIE 115 adhere to surfaces coated with fibronectin or laminin and extend rapidly growth cone-containing neurites. Some lectin-coate...

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Bibliographic Details
Published inEuropean journal of cell biology Vol. 35; no. 1; p. 55
Main Authors Jousimaa, J, Merenmies, J, Rauvala, H
Format Journal Article
LanguageEnglish
Published Germany 01.09.1984
Subjects
Animals
Axons - ultrastructure
Cell Adhesion
Cell Differentiation
Cells, Cultured
Fibronectins - physiology
Laminin - physiology
Lectins
Neuroblastoma - ultrastructure
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Summary:A method using proteins covalently coupled to glass surfaces has been applied to studies on the differentiation of neuroblasts. Neuroblastoma cells from clones N 18 and NIE 115 adhere to surfaces coated with fibronectin or laminin and extend rapidly growth cone-containing neurites. Some lectin-coated surfaces are also able to support neurite outgrowth, although the activities are lower than those of fibronectin and laminin. We discuss the biochemical requirements of the surface structures capable of inducing a differentiated neuronal morphology to neuroblastoma cells, and also consider the possible relationship of the results to physiological differentiation phenomena.
ISSN:0171-9335