Uptake of reverse T3 in the human choriocarcinoma cell line, JAr

• Published in: Placenta (Eastbourne) Vol. 20; no. 1; pp. 65 - 70
• Main Authors: MITCHELL, A. M, MANLEY, S. W, ROWAN, K. A, MORTIMER, R. H
• Format: Journal Article
• Language: English
• Published: Oxford Elsevier 1999
• Subjects: Biological and medical sciences; Cell physiology; Choriocarcinoma - metabolism; Female; Fundamental and applied biological sciences. Psychology; Humans; Iodine Radioisotopes; Kinetics; Membrane and intracellular transports; Molecular and cellular biology; Pregnancy; Thyroxine - pharmacology; Triiodothyronine - pharmacology; Triiodothyronine, Reverse - metabolism; Tumor Cells, Cultured; Uterine Neoplasms - metabolism; Human; Trophoblaste pathology; Pregnancy disorders; Biological transport; Malignant tumor; Placenta diseases; Secretory tumor; Biological fixation; Regulation(control); Placenta; Established cell line; Chorioepithelioma; Thyroid hormone; Triiodothyronine; Diffusion; Tumor cell
• ISSN: 0143-4004; 1532-3102
• DOI: 10.1053/plac.1999.0340

The uptake and efflux of reverse triiodothyronine (rT3) in JAr cells were investigated. Uptake of 125I-rT3 was time dependent and reversible with a saturable component of around 70 per cent of total uptake after 30 min of incubation. Efflux was not saturable. Kinetic analysis of the initial specific uptake rates revealed an uptake process with a Michaelis constant of 3.04+/-0.53 microM (mean+/-SEM, n=15) and a corresponding maximum velocity of 9.65+/-2.49 pmol/min/mg protein (n=15). Uptake of rT3 was stereospecific, but not specific for rT3, as unlabelled L stereoisomers of thyroid hormone analogues were more effective as inhibitors of 125I-rT3 uptake than rT3. Unlabelled T3 and thyroxine (T4) (10 microM) reduced cellular uptake of 125I-rT3 by around 82 and 74 per cent, respectively. The calculated inhibition constants Ki were 1.23+/-0.29 microM (n=4) and 0.66+/-0.19 microM (n=4) for T3 and T4, respectively. Similarly, rT3 reduced cellular uptake of 125I-T3 and 125I-T4 by 34 and 23 per cent, respectively. The calculated inhibition constants Ki were 1.75+/-0.55 microM (n=8) and 1.08+/-0.36 microM (n=8) for the inhibition of 125I-T3 and 125I-T4 uptake, respectively. Reverse T3 inhibited efflux of 125I-T3 from the cells by around 20 per cent, but did not inhibit efflux of 125I-T4. These results suggest that uptake of rT3 in JAr cells may occur via a single, saturable membrane carrier, which also interacts with T3 and T4, while efflux of rT3 may occur by passive diffusion.