The in vitro culture of Onchocerca spp.: I. Preliminary observations on the development of the second to the third stage larvae

Second stage larvae of Onchocerca lienalis and O. gutturosa were induced to moult successfully to the third stage in vitro. Following development in their vectors Simulium ornatum s.l. and Culicoides nubeculosus the second stage larvae were kept in a cell free culture system. At least 25% of the sec...

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Bibliographic Details
Published inApplied parasitology Vol. 36; no. 1; p. 47
Main Authors Hagen, H E, Grunewald, J, Ham, P J
Format Journal Article
LanguageEnglish
Published Germany 01.02.1995
Subjects
Animals
Cattle
Cattle Diseases
Ceratopogonidae - parasitology
Diptera - parasitology
Insect Vectors
Larva
Onchocerca - growth & development
Onchocerca - isolation & purification
Onchocerciasis - parasitology
Onchocerciasis - veterinary
Species Specificity
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Summary:Second stage larvae of Onchocerca lienalis and O. gutturosa were induced to moult successfully to the third stage in vitro. Following development in their vectors Simulium ornatum s.l. and Culicoides nubeculosus the second stage larvae were kept in a cell free culture system. At least 25% of the second stage larvae of either Onchocerca species completed the moult successfully although no feeder layers had been used. In the absence of CO2 (5% in air) the number of the second stage larvae moulting successfully to the infective stage dropped to 6 and 9% respectively.
ISSN:0943-0938